Compact biosensor for non-invasive glucometer
Millions of people worldwide suffer from diabetes, especially type 1 every day 2-3 times a special lancet puncture the finger to obtain a drop of blood for glucose level analysis. This method is inconvenient, fraught with pain and, more importantly, can lead to infection by numerous punctures.
"Several" Ltd. is nearing to complete the work on the creation of a fundamentally new technique, which is based on the analysis not of blood, but of the sugar concentration in tears, urine, saliva, sweat. Existing glucometers, even the most modern are not sensitive enough to detect such small concentrations of sugar - in human biological fluids glucose contained approximately 50-100 times less than the same amount of blood.
Project participants developed a compact biosensor for non-invasive glucose monitoring in biological fluids of people. The principle of operation of the system is based on the detection of the interaction metal dielectric structures (nanoparticles) possesses the effect of the localized plasmon resonance, and the glucose- binding proteins which act as the sensing element of the biosensor, and have a high specificity to glucose. So far, the team has developed the applicants carrier substrate of biosensor with silver nanoparticles on the surface and in the subsurface layer of glass of special composition), with the formation of the waveguide layer with varying thickness. The technique of immobilization on the substrate surface of the carrier with silver NP glucose- binding protein and in vitro performance of the biosensor shown glucose sensitivity 0.2 mg / ml, which exceeds the commercially available analogs. The apparatus can be used in determining the level of glucose in the patients saliva, urine, sweat, and tears. The emergence of non-invasive blood glucose meter on the market is planned in early 2017.
Methods fixing the silver nanoparticles to the surface of glass by means of a biocompatible polymer allowing it to set thickness within 10-200 nm, which is used to enhance the fluorescence of biological objects low emissivity and promising to create biosensors for diagnosis of conformational diseases at early stages. Choose the appropriate sensitive element can detect the presence of analytes in body fluids of cancer markers, different types of hepatitis and other viral diseases.